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Yeasen Biotechnology tunel staining
Tunel Staining, supplied by Yeasen Biotechnology, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/tunel+staining/assay+kit+ros/pm42310754-136-1-7
Average 86 stars, based on 1 article reviews
tunel staining - by Bioz Stars, 2026-10
86/100 stars

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Related Articles

TUNEL Assay:

Article Title: Vitamin A-coupled MSCs- derived extracellular vesicles relieve acute liver injury by targeting hepatic stellate cells to deliver REDD1.
Article Snippet: .. For TUNEL staining, TUNEL assay kit (40307ES60, Yeasen, Shanghai, China) was performed, and DAPI was used to stain the cell slides fixed with 4% paraformaldehyde fixation solution. ..

Article Title: Regenerating myofiber with activating of TGF-β signaling contributes to macrophage efferocytosis through enhancing Tregs response in inflamed muscle
Article Snippet: To assess the uptake of exogenous apoptotic cells by macrophages in inflamed muscle, neutrophils were isolated from the spleen of B6 mice using density gradient centrifugation with Ficoll Plus 1.083 (P4360, Solarbio, China). .. Apoptosis was induced by UV irradiation (254 nm, 30 minutes), and apoptotic cells were labeled with the membrane-intercalating dye DiD (C1039) or assessed by TUNEL staining (40306ES60, Yeasen, China). ..

Article Title: Non-viral Gene Therapy for Melanoma Using Lysenin from Eisenia Foetida.
Article Snippet: .. TUNEL staining of tumor tissues was carried out according to the manufacturer’s instructions (YEASEN, Shanghai, China). ..

Article Title: Centipede Polypeptide Affects the Inflammatory Reaction and Ferroptosis of Liver Cancer Cells Through the p53/TRAIL Pathway.
Article Snippet: .. TUNEL staining (40306ES50, Yeasen, China) was applied to assay the apoptosis rate of HepG2 cells in tumour tissues [40]. ..

Article Title: Centipede Polypeptide Affects the Inflammatory Reaction and Ferroptosis of Liver Cancer Cells Through the p53/ TRAIL Pathway
Article Snippet: .. TUNEL staining (40306ES50, Yeasen, China) was applied to assay the apoptosis rate of HepG2 cells in tumour tissues [ ]. ..

Article Title: 5-Azacytidine inhibits endoplasmic reticulum stress and apoptosis of nucleus pulposus cells by preserving PPARγ via promoter demethylation.
Article Snippet: Low back pain (LBP) is a common symptom of intervertebral disc degeneration (IDD).. However, the pathogenesis of IDD is not well understood.. Several studies have shown that patients with IDD experience aberrant changes in DNA methylation.

Article Title: RP1 Dual-AAV Gene Therapy Preserves Retinal Structure and Ameliorates Photoreceptor Degeneration in a Murine Model of Retinitis Pigmentosa
Article Snippet: .. TUNEL staining was performed strictly according to the instructions (40307ES; Yeasen, Shanghai, China). .. Finally, the slides were counterstained for 5 minutes with DAPI (62248; Invitrogen, Carlsbad, CA, USA), sealed with mounting medium, and imaged under a confocal laser microscope (Nikon, Tokyo, Japan).

Staining:

Article Title: Vitamin A-coupled MSCs- derived extracellular vesicles relieve acute liver injury by targeting hepatic stellate cells to deliver REDD1.
Article Snippet: .. For TUNEL staining, TUNEL assay kit (40307ES60, Yeasen, Shanghai, China) was performed, and DAPI was used to stain the cell slides fixed with 4% paraformaldehyde fixation solution. ..

Article Title: Regenerating myofiber with activating of TGF-β signaling contributes to macrophage efferocytosis through enhancing Tregs response in inflamed muscle
Article Snippet: To assess the uptake of exogenous apoptotic cells by macrophages in inflamed muscle, neutrophils were isolated from the spleen of B6 mice using density gradient centrifugation with Ficoll Plus 1.083 (P4360, Solarbio, China). .. Apoptosis was induced by UV irradiation (254 nm, 30 minutes), and apoptotic cells were labeled with the membrane-intercalating dye DiD (C1039) or assessed by TUNEL staining (40306ES60, Yeasen, China). ..

Article Title: Non-viral Gene Therapy for Melanoma Using Lysenin from Eisenia Foetida.
Article Snippet: .. TUNEL staining of tumor tissues was carried out according to the manufacturer’s instructions (YEASEN, Shanghai, China). ..

Article Title: Centipede Polypeptide Affects the Inflammatory Reaction and Ferroptosis of Liver Cancer Cells Through the p53/TRAIL Pathway.
Article Snippet: .. TUNEL staining (40306ES50, Yeasen, China) was applied to assay the apoptosis rate of HepG2 cells in tumour tissues [40]. ..

Article Title: Centipede Polypeptide Affects the Inflammatory Reaction and Ferroptosis of Liver Cancer Cells Through the p53/ TRAIL Pathway
Article Snippet: .. TUNEL staining (40306ES50, Yeasen, China) was applied to assay the apoptosis rate of HepG2 cells in tumour tissues [ ]. ..

Article Title: 5-Azacytidine inhibits endoplasmic reticulum stress and apoptosis of nucleus pulposus cells by preserving PPARγ via promoter demethylation.
Article Snippet: Low back pain (LBP) is a common symptom of intervertebral disc degeneration (IDD).. However, the pathogenesis of IDD is not well understood.. Several studies have shown that patients with IDD experience aberrant changes in DNA methylation.

Article Title: RP1 Dual-AAV Gene Therapy Preserves Retinal Structure and Ameliorates Photoreceptor Degeneration in a Murine Model of Retinitis Pigmentosa
Article Snippet: .. TUNEL staining was performed strictly according to the instructions (40307ES; Yeasen, Shanghai, China). .. Finally, the slides were counterstained for 5 minutes with DAPI (62248; Invitrogen, Carlsbad, CA, USA), sealed with mounting medium, and imaged under a confocal laser microscope (Nikon, Tokyo, Japan).

Irradiation:

Article Title: Regenerating myofiber with activating of TGF-β signaling contributes to macrophage efferocytosis through enhancing Tregs response in inflamed muscle
Article Snippet: To assess the uptake of exogenous apoptotic cells by macrophages in inflamed muscle, neutrophils were isolated from the spleen of B6 mice using density gradient centrifugation with Ficoll Plus 1.083 (P4360, Solarbio, China). .. Apoptosis was induced by UV irradiation (254 nm, 30 minutes), and apoptotic cells were labeled with the membrane-intercalating dye DiD (C1039) or assessed by TUNEL staining (40306ES60, Yeasen, China). ..

Labeling:

Article Title: Regenerating myofiber with activating of TGF-β signaling contributes to macrophage efferocytosis through enhancing Tregs response in inflamed muscle
Article Snippet: To assess the uptake of exogenous apoptotic cells by macrophages in inflamed muscle, neutrophils were isolated from the spleen of B6 mice using density gradient centrifugation with Ficoll Plus 1.083 (P4360, Solarbio, China). .. Apoptosis was induced by UV irradiation (254 nm, 30 minutes), and apoptotic cells were labeled with the membrane-intercalating dye DiD (C1039) or assessed by TUNEL staining (40306ES60, Yeasen, China). ..

Membrane:

Article Title: Regenerating myofiber with activating of TGF-β signaling contributes to macrophage efferocytosis through enhancing Tregs response in inflamed muscle
Article Snippet: To assess the uptake of exogenous apoptotic cells by macrophages in inflamed muscle, neutrophils were isolated from the spleen of B6 mice using density gradient centrifugation with Ficoll Plus 1.083 (P4360, Solarbio, China). .. Apoptosis was induced by UV irradiation (254 nm, 30 minutes), and apoptotic cells were labeled with the membrane-intercalating dye DiD (C1039) or assessed by TUNEL staining (40306ES60, Yeasen, China). ..

other:

Article Title: PLOD1 promotes the malignancy of hepatocellular carcinoma by facilitating the NF-κB/IL-6/STAT3-dependent TCA cycle
Article Snippet: Apoptosis and cell cycle assay For the TUNEL assay, HCC cells were seeded into co-focal dishes (2×105 cells per well) and cultured for 48 h. Cells were fixed with 4% paraformaldehyde in PBS for 30 min at room temperature and processed for TUNEL (YEASEN) staining according to the manufacturer’s instructions.



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TSF and Rg3 regulate HDAC8/BRCA2/DRP1 pathway inhibits DOX‐induced <t>cardiomyocyte</t> <t>apoptosis.</t> (a) Apoptosis was assessed using <t>TUNEL</t> staining (Scale bar = 50 μm). (b) The results of western blotting for each group. (c) The expression of Bcl‐2 for each group. (d) The expression of DRP1 for each group. (e) The expression of HDAC8 for each group. (f) The expression of BRCA2 for each group. (g) Immunofluorescence staining for observing the colocalization of HDAC8, BRCA2, and DRP1 (Scale bar = 1 mm). Data are expressed as mean ± standard from three individual experiments. Data analysis among groups was performed using ANOVA, with LSD for equal variances and Dunnett’s‐T3 for unequal variances, with significance levels of * p < 0.05, ** p < 0.01, *** p < 0.001.
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Image Search Results


TSF and Rg3 regulate HDAC8/BRCA2/DRP1 pathway inhibits DOX‐induced cardiomyocyte apoptosis. (a) Apoptosis was assessed using TUNEL staining (Scale bar = 50 μm). (b) The results of western blotting for each group. (c) The expression of Bcl‐2 for each group. (d) The expression of DRP1 for each group. (e) The expression of HDAC8 for each group. (f) The expression of BRCA2 for each group. (g) Immunofluorescence staining for observing the colocalization of HDAC8, BRCA2, and DRP1 (Scale bar = 1 mm). Data are expressed as mean ± standard from three individual experiments. Data analysis among groups was performed using ANOVA, with LSD for equal variances and Dunnett’s‐T3 for unequal variances, with significance levels of * p < 0.05, ** p < 0.01, *** p < 0.001.

Journal: Food Science & Nutrition

Article Title: Exploring the Mechanisms of Total Saponins of Black Ginseng and Ginsenoside Rg3 Against Doxorubicin‐Induced Cardiotoxicity

doi: 10.1002/fsn3.71968

Figure Lengend Snippet: TSF and Rg3 regulate HDAC8/BRCA2/DRP1 pathway inhibits DOX‐induced cardiomyocyte apoptosis. (a) Apoptosis was assessed using TUNEL staining (Scale bar = 50 μm). (b) The results of western blotting for each group. (c) The expression of Bcl‐2 for each group. (d) The expression of DRP1 for each group. (e) The expression of HDAC8 for each group. (f) The expression of BRCA2 for each group. (g) Immunofluorescence staining for observing the colocalization of HDAC8, BRCA2, and DRP1 (Scale bar = 1 mm). Data are expressed as mean ± standard from three individual experiments. Data analysis among groups was performed using ANOVA, with LSD for equal variances and Dunnett’s‐T3 for unequal variances, with significance levels of * p < 0.05, ** p < 0.01, *** p < 0.001.

Article Snippet: Cardiac apoptosis was assessed using TUNEL staining, following the manufacturer's guidelines (Servicebio, China).

Techniques: TUNEL Assay, Staining, Western Blot, Expressing, Immunofluorescence